2017-05-16 10:38:472025-05-24 12:49:46

locus

BSU28760

BSU_28760

geneLength

1182

1185

outlinks

bsu

BSU28760

BSU_28760

Gene

Coordinates

2,943,006 → 2,944,190

2,943,006 2,944,190

The protein

Catalyzed reaction/ biological activity

sn-glycerol-1-phosphate + NAD(P)⁺ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)

NAD⁺ + sn-glycerol 1-phosphate --> dihydroxyacetone phosphate + H⁺ + NADH (according to UniProt)

NADP⁺ + sn-glycerol 1-phosphate --> dihydroxyacetone phosphate + H⁺ + NADPH (according to UniProt)

The protein

Protein family

glycerol-1-phosphate dehydrogenase family (according to Swiss-Prot)

glycerol-1-phosphate dehydrogenase family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[protein|AroB ]] 36/154 (23%)

[[this]]

The protein

Structure

[PDB|3CE9] (from '' Clostridium Acetobutylicum'' 74/241(31%) )

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cytoplasm (according to UniProt)

Biological materials

Mutant

MGNA-A985 (araM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/985 NBRP B. subtilis, Japan]

BKE28760 ([[gene|araM]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC, downstream forward: _UP4_TAGCCCGCACCTCGAATGGA

BKK28760 ([[gene|araM]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAACGTCAGCTGCGATAC, downstream forward: _UP4_TAGCCCGCACCTCGAATGGA

The protein

[SW|Cofactors]

NADH (according to UniProt)